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KMID : 0380020110260010069
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Korean Journal of Biotechnology and Bioengineering
2011 Volume.26 No. 1 p.69 ~ p.77
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Production of Gemcitabine-Loaded Poly (L-lactic acid) Microparticles Using Supercritical Carbon Dioxide: Effect of Process Parameters
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Joo Hyun-Jae
Jung In-Il
Lim Kyo-Bin
Ryu Jong-Hoon
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Abstract
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In this study, poly (L-lactic acid) (PLLA) microparticles containing gemcitabine hydrochloride were prepared by a supercritical fluid process, called aerosol solvent extraction system (ASES), utilizing supercritical carbon dioxide as antisolvent. The influence of process parameters such as temperature, pressure, CO2 and solution flow rate, solution concentration, and feed ratio of drug to polymer on the morphology and characteristics of the microparticles was studied in detail. The gemcitabine-loaded microparticles exhibited a spherical shape with a smooth surface. The entrapment efficiency of gemcitabine increased with increasing temperature, solution concentration and CO2 flow rate and with decreasing drug/polymer feed ratio. The maximum drug loading obtained from the ASES process was found to be about 11%. The ASES-processed PLLA microparticles containing gemcitabine showed a relatively high initial burst due to the presence of surface pores on the microparticles and the poor affinity between drug and polymer.
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KEYWORD
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gemcitabine, poly (L-lactic acid), supercritical carbon dioxide, microparticle, ASES
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